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KMID : 0881720130280020188
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Journal of Food Hygiene and Safety
2013 Volume.28 No. 2 p.188 ~ p.193
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Development and Application of Detection Method for Aphanizomenon flos-aquae not Usable as a Food Materials in Korea
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Park Yong-Chjun
Shin Seung-Jung
Lee Ho-Yeon
Kim Young-Sang
Kim Mi-Ra
Lee Sang-Jae
Lee Hwa-Jung
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Abstract
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Anatoxin-a, saxitoxin and neosaxitoxin are produced by Aphanizomenon flos-aquae that is a sort of the cyanobacteria phylum. Therefore, it is not permitted for food materials in Korea. Traditionally, the classification of cyanobacteria has been based on morphological characters such as trichome width, cell size, division planes, shape, and the presence of character such as gas vacuole. But, some diagnostic features, such as gas vacuole or akinetes, can show variation with different environmental or growth conditions and even be lost during cultivation. Therefore, we developed detection method for functional foods containing Aph. flos-aquae by PCR. To design the primer, 16S rRNA region of Aph. flos-aquae, Spirulina laxissima, and Spirulina spp. registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for comparative analysis, BioEdit ver. 7.0.9.0. was used. As a result, we was design AFA-F1/AFAR1 (363 bp) primer for the differentiation Aph. flos-aquae from chlorella, spirulina, green tea, and spinach. Also, it could be distinguished chlorella and spirulina products those are made to contain 1% Aph. flos-aquae.
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KEYWORD
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Aphanizomenon flos-aquae, 16S rRNA, PCR
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